Bioethanol Production from Chlorella Pyrenoidosa by Using Enzymatic Hydrolysis and Fermentation Method

Yerizam, Muhammad and Jannah, Asyeni Miftahul and Aprianti, Nabila (2022) Bioethanol Production from Chlorella Pyrenoidosa by Using Enzymatic Hydrolysis and Fermentation Method. Journal of Ecological Engineering. pp. 34-40. ISSN 2299–8993

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Abstract

Starch can be found in microalgae, the raw material for the third generation of bioethanol production. One of them is C. pyrenoidosa. This study was conducted to analyze the efect o α-amylase enzyme concentration on the glucose contents produced and the efects o ermentation time on bioethanol contents produced. The hydrolysis process o this study was conducted using an α-amylase enzyme produced by A. niger. Several analyses in this research were carried out, including the analysis of enzyme activity using the Fuwa method, the analysis of glucose levels from enzymatic hydrolysis using the DNS method, and the analysis of bioethanol contents using the density method and GC-MS. The highest glucose content was 0.67 mg/mL, which was obtained from the addition o 40% (v/w) α-amylase enzyme, and the yield o bioethanol content rom the sample treated 40% (v/w) α-amylase enzyme and fermented for 9 days was the optimum, which produced 28.07% of bioethanol content.

Item Type: Article
Uncontrolled Keywords: Chlorella pyrenoidosa, hydrolysis, Enzyme α-amylase, glucose, ermentation, bioethanol
Subjects: T Technology > TP Chemical technology
Divisions: Chemical Engineering > Journal
Depositing User: Mrs Trisni Handayani
Date Deposited: 14 Apr 2023 01:58
Last Modified: 14 Apr 2023 01:58
URI: http://eprints.polsri.ac.id/id/eprint/13342

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